Open AccessHigh level production of Echerichia coli outer membrane proteins OmpA and OmpF intracellularly in Bacillus subtilis
Author(s) -
Puohiniemi Ritvaleena,
Butcher Sarah,
Tarkka Eveliina,
Sarvas Matti
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04383.x
Subject(s) - bacillus amyloliquefaciens , bacillus subtilis , escherichia coli , bacterial outer membrane , biology , membrane protein , centrifugation , yield (engineering) , signal peptide , guanidine , biochemistry , chemistry , bacteria , membrane , microbiology and biotechnology , recombinant dna , fermentation , gene , genetics , materials science , metallurgy
A high yield of Escherichia coli outer membrane proteins OmpA (about 200 mg/l) and OmpF (about 100 mg/l) was obtained in Bacillus subtilis when produced intracellularly. The yield was more than 100‐fold higher than the yield of these proteins by a similar vector containing the complete signal sequence of α‐amylase of B. amyloliquefaciens . Both proteins isolated after breakage of the B. subtilis cells by low‐speed centrifugation were about 70% pure and could be solubilized by Sarkosyl, SDS and guanidine hydrochloride.