Open AccessSecretion of oligomeric Val 8 ‐human calcitonin by Saccharomyces cerevisiae
Author(s) -
Mironova Rumjana,
Philipova Doranelly,
Marekov Lyuben,
Venkov Pencho,
Ivanov Ivan
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04382.x
Subject(s) - saccharomyces cerevisiae , recombinant dna , yeast , mutant , secretion , plasmid , biology , microbiology and biotechnology , wild type , biochemistry , gene
Monomeric human calcitonin (hCT) gene and oligomeric hCT genes composed of two, three or four head‐to‐tail linked monomers were fused in‐frame to the yeast α‐factor leader coding sequence and inserted into expression vectors. Isogenic wild‐type and fragile mutant Saccharomyces cerevisiae strains were transformed with the constructed plasmids and the yield of recombinant protein secreted into the culture medium was measured. The yeast cells secreted equal (molar) amounts of all of the hCT variants. The recombinant proteins remained stable in the growth medium for at least 3 days. The fragile cells secreted about 30% more hCT as compared to the wild‐type yeast cells.