Open AccessIdentification of an insertion sequence, IS1081, in Mycobacterium bovis
Author(s) -
Collins Desmond M.,
Stephens Diana M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04380.x
Subject(s) - insertion sequence , mycobacterium bovis , biology , transposase , polymerase chain reaction , mycobacterium tuberculosis , genetics , whole genome sequencing , genome , transposable element , mycobacterium tuberculosis complex , sequence (biology) , virology , microbiology and biotechnology , tuberculosis , gene , medicine , pathology
An insertion sequence, IS1081, in the genome of Mycobacterium bovis has been identified and sequenced. It is 1324 bp long with 15 bp inverted repeat ends and contains a large ORF. There are six copies of IS1081 in the genome of M. bovis and the element is also present in Mycobacterium tuberculosis . IS1081 is not closely related to other DNA elements described in actinomycetes but its putative transposase bears some resemblance to that of IS256 from Staphylococcus aureus . IS1081 may be useful for genetic manipulations and for developing a diagnostic test for bovine tuberculosis based on the polymerase chain reaction.