Open AccessProline auxotrophic and catabolic mutants of Rhizobium leguminosarum biovar viciae strain C1204b are unaffected in nitrogen fixation *
Author(s) -
Chien ChingTe,
Rupp Richard,
Beck Sidney,
Orser Cindy S.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04365.x
Subject(s) - rhizobium leguminosarum , pisum , biology , nitrogen fixation , proline , sativum , nitrogenase , biochemistry , microbiology and biotechnology , strain (injury) , rhizobium , rhizobiaceae , mutant , biovar , bacteria , symbiosis , botany , amino acid , genetics , anatomy , gene
Summary The potential role of proline in bacteroid metabolism of Rhizobium leguminosarum biovar viciae strain C1204b was investigated. Proline auxotrophic, catabolic, and overproducing mutants were generated and used as inoculants of pea ( Pisum sativum L. cv Alaska). Cytosolic and bacteroid levels of glutamate and proline determined for the nodular tissue did not support the role of proline as a key metabolite supplied by the host. Furthermore, a R. leguminosarum proline catabolic mutant had a negligible impact on nitrogenase activity as evaluated by acetylene reduction.