Open AccessLevels of activity of enzymes involved in anaerobic utilization of sugars by six yeast species: observations towards understanding the Kluyver effect
Author(s) -
Sims A.P.,
Barnett J.A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04364.x
Subject(s) - pyruvate decarboxylase , yeast , biochemistry , alcohol dehydrogenase , glycoside hydrolase , saccharomyces cerevisiae , enzyme , carboxy lyases , anaerobic exercise , pyruvate dehydrogenase complex , glycoside , dehydrogenase , chemistry , biology , stereochemistry , physiology
Summary The activities of pyruvate decarboxylase, alcohol dehydrogenase and certain glycosidases were measured for six species of yeast. Five of these yeasts could utilize one or more disaccharides aerobically, but not anaerobically, although all could use d ‐glucose anaerobically. That is, each of the five showed the Kluyver effect; but the sixth yeast, Saccharomyces cerevisiae , did not do so. When grown on a glycoside with which it gave the Kluyver effect, each yeast had much less pyruvate decarboxylase activity than when grown on d ‐glucose or another glycoside. There was no consistent corresponding lowering of activity of either alcohol dehydrogenase, or of the appropriate glycosidase. Hence, pyruvate decarbocylase may have a role in producing the Kluyver effect.