Open AccessAnalysis of Bordetella pertussis cya operon regulation by use of cya‐lac fusions
Author(s) -
Goyard Sophie,
Ullmann Agnes
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04357.x
Subject(s) - bordetella pertussis , operon , escherichia coli , microbiology and biotechnology , adenylate cyclase toxin , lac operon , pertussis toxin , virulence , bordetella , gene , biology , locus (genetics) , chemistry , bacteria , genetics , g protein , signal transduction
Summary In Bordetella pertussis virulence‐associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg ‐locus. We have constructed cya‐lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli , neither is it activated by bvg , when introduced in trans . The cya‐lac fusion is fully active when returned to B. pertussis by homologous recombination and responds to bvg ‐dependent activation and environmental regulation. These results indicate that in B. pertussis the activation of the cya operon by bvg is indirect.