Open AccessIsolation and characterization of maltose non utilizing ( mnu ) mutants mapping outside the MAL1 locus in Saccharomyces cerevisiae *
Author(s) -
Vai Marco,
Goldenthal Michael J.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04354.x
Subject(s) - maltose , maltase , lactose permease , saccharomyces cerevisiae , mutant , biology , locus (genetics) , gene , biochemistry , raffinose , genetics , permease , sucrose , enzyme
Summary The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization. They include regulatory, maltose transport and maltase genes designated MAL1R, MAL1T and MAL1S respectively. Using a MAL1 strain transformed with an episomal, multicopy plasmid carrying the MAL2 locus, five recessive and one dominant mutant unable to grow on maltose, but still retaining a functional MAL1 locus were isolated. All the mutants could use glycerol, ethanol, raffinose and sucrose as a sole carbon source; expression of the maltase and maltose permease genes was severely and coordinately reduced. Only the dominant mutant failed to accumulate the MAL1R mRNA.