Open AccessEffects of culture conditions on expression of the ice nucleation phenotype of Pseudomonas syringae
Author(s) -
Pooley Linda,
Brown Terence A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04353.x
Subject(s) - pseudomonas syringae , nucleation , phenotype , ice nucleus , strain (injury) , pseudomonadaceae , pseudomonadales , agar plate , agar , exponential growth , biology , stationary phase , bacteria , chemistry , microbiology and biotechnology , pseudomonas , biochemistry , genetics , gene , chromatography , physics , organic chemistry , quantum mechanics , anatomy
Summary Pseudomonas syringae 1105 was grown under a variety of culture conditions and the effects on ice nucleation determined. Growth in a defined medium, Koser citrate broth, resulted in a greater number of more active ice nuclei than growth in either of three complex media. The ice nucleation phenotype was not expressed uniformly during the growth cycle in broth culture. Instead a maximal level of ice nucleation was observed during the middle to exponential phase and the phenotype was not expressed in stationary phase. Cells grown on agar plates expressed the phenotype to a much greater extent than cells from broth cultures.