Open AccessRapid characterization of Mycobacterium fortuitum‐chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes
Author(s) -
Kanaujia G.V.,
Katoch V.M.,
Shivannavar C.T.,
Sharma V.D.,
Patil M.A.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04348.x
Subject(s) - mycobacterium fortuitum , ecori , restriction fragment length polymorphism , restriction enzyme , ribosomal rna , biology , agarose gel electrophoresis , genomic dna , microbiology and biotechnology , terminal restriction fragment length polymorphism , ribosomal dna , restriction fragment , gene , 5s ribosomal rna , dna , genetics , mycobacterium , polymerase chain reaction , bacteria , 18s ribosomal rna , phylogenetics
Summary Using labelled, γ‐ 32 P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum‐chelonei complex was analysed. Each DNA sample was cleaved with Eco RI restriction endonuclease, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose membrane. Fragments of DNA containing rRNA genes were identified by hybridization with γ‐ 32 P‐labelled rRNA. Patterns were found to be species specific and both the species were distinguishable from each other. Results indicate that this approach can be used for rapid genomic characterization of the Mycobacterium fortuitum‐chelonei complex.