Open AccessSequence of the 5S rRNA gene and organization of ribosomal RNA operons in Streptomyces rimosus
Author(s) -
Plohl Miroslav,
Gamulin Vera
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04337.x
Subject(s) - operon , ribosomal rna , biology , 23s ribosomal rna , genetics , gal operon , l arabinose operon , gene , 5s ribosomal rna , microbiology and biotechnology , rna , 18s ribosomal rna , ribosome , escherichia coli
Summary One Streptomyces rimosus ribosomal RNA gene set ( rrnF ) was cloned into pBR322 as 8.8 kilobase‐pairs Bam HI fragment and sequence of the 3′ end of the operon with 5S rRNA gene was determined. 5S rRNA gene is 120 bp long and highly homologous with two other known 5S rRNA from Streptomyces . Remarkable sequence homology with S. ambofaciens rrnD operon exists in the 3′ noncoding regions, including first transcription termination signal. S. rimosus rrnF operon contains second putative terminator which is absent in S. ambofaciens rrnD operon. tRNA genes were not found at the 3′ end of rrnF operon. The number of rRNA operons in S. rimosus was defined by Southern hybridization analysis. S. rimosus possess six rRNA operons and all rRNA operons contain identical Pst I fragment of 4.5 kb. rRNA genes in operons are separated by short intergenic regions and organized in the order 16S–23S–5S. Efforts to connect tRNA genes with S. rimosus rRNA operons were unsuccessful.