Open AccessHyperinduction of an aliphatic nitrilase by Rhodococcus rhodochrous K22
Author(s) -
Kobayashi Michihiko,
Yanaka Noriyuki,
Nagasawa Toru,
Yamada Hideaki
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04333.x
Subject(s) - nitrilase , rhodococcus rhodochrous , rhodococcus , chemistry , urea , amine gas treating , sorbitol , yeast extract , yeast , biochemistry , organic chemistry , enzyme , fermentation
Summary We investigated the optimum conditions for the formation of a nitrilase, which acts on aliphatic nitriles, by Rhodococcus rhodochrous K22 and developed a growth medium containing isovaleronitrile, sorbitol, yeast extract, NZ amine and urea, and produced organisms containing approximately 20% of their total soluble protein as nitrilase.