Open AccessA gene in Schizosaccharomyces pombe analogous to the RAD4 Gene of Saccharomyces cerevisiae
Author(s) -
Choi In Soon,
Kim Jae Bum,
Hong Seung Hwan,
Park Sang
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04328.x
Subject(s) - schizosaccharomyces pombe , saccharomyces cerevisiae , biology , gene , southern blot , cyclin dependent kinase 7 , genomic dna , microbiology and biotechnology , genetics , northern blot , schizosaccharomyces , gene cluster , dna , restriction map , rna , plasmid , cell cycle , cyclin dependent kinase 2
Summary A gene, analogous to the RAD4 gene, which is required for nucleotide excision repair in Saccharomyces cerevisiae , also exists in Schizosaccharomyces pombe . RNA isolated from wild type S. pombe cells strongly cross‐hybridized with the 1.2 kb Pvu II DNA fragment of the S. cerevisiae RAD4 genomic clone (pPCl). Chromosomal DNA isolated from S. pombe had similar restriction patterns to those from S. cerevisiae , as determined by Southern blot analysis. Two species of mRNA, 3.1 and 1.8 kb, were identified by Northern hybridization. The level of these transcripts did not increase upon UV‐irradiation, suggesting that the RAD4 ‐like gene in S. pombe is not UV‐inducible.