Open AccessPhysiology and dinitrogen fixation of Acetobacter diazotrophicus
Author(s) -
Stephan M.P.,
Oliveira M.,
Teixeira K.R.S.,
MartinezDrets G.,
Döbereiner J.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04323.x
Subject(s) - nitrogenase , nitrogen fixation , gluconic acid , acetobacter , chemistry , biochemistry , browning , ammonium , food science , extracellular , nitrogen , fermentation , organic chemistry
Summary The recently described species Acetobacter diazotrophicus isolated from sugar cane roots and stems was found capable of growth at pH 3.0 and showed high nitrogenase activity even at pH 2.5. No growth occurred at pH 7.5. Extracellular oxidation of glucose followed by gluconic acid formation was necessary for initiation of logarithmic growth, which proceeded with N 2 as the sole nitrogen source. N 2 ‐dependent growth did not occur in N‐free liquid medium under air, but starter doses of 0.6 to 1 mM (NH 4 ) 2 SO 4 led to active N 2 ‐fixing cultures after 34 h. Nitrogenase activity was only partially inhibited by 20 mM (NH 4 + ) 2 SO 4 and several amino acids showed similar effects. However, NO 3 − did not inhibit or repress nitrogenase activity.