Open AccessConstruction of a family of lactococcal vectors for gene cloning and translational fusion
Author(s) -
Xu Fengfeng,
Pearce Lindsay E.,
Yu PakLam
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04321.x
Subject(s) - cloning (programming) , replicon , cloning vector , genetics , biology , escherichia coli , gene , multiple cloning site , lac operon , molecular cloning , vector (molecular biology) , fusion protein , computational biology , microbiology and biotechnology , plasmid , recombinant dna , peptide sequence , computer science , programming language
Summary A family of stable lactococcal vectors have been constructed based on the pFX1 replicon using either the α fragment or the complete Escherichia coli lacZ gene as a selective marker. These vectors also incorporate multiple cloning sites and examples are given of their use for gene cloning and translational fusion studies in lactococci.