Open AccessLocalization of actin and characterization of its isoforms in the hyphae of Neurospora crassa
Author(s) -
Barja Francisco,
Thi BichNga Nguyen,
Turian Gilbert
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04315.x
Subject(s) - neurospora crassa , phalloidin , hypha , biology , actin , actin binding protein , organelle , gene isoform , biochemistry , microbiology and biotechnology , actin cytoskeleton , cytoskeleton , gene , mutant , cell
Summary The actin of Neurospora crassa wild type Strain St. Lawrence has been purified, characterized and localized. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I‐Sepharose. This protein was identified as actin on immunoblots when an anti‐actin monoclonal antibody raised against chicken gizzards was used as a probe. After two‐dimensional gel electrophoresis three actin isoforms were detected. The distribution of actin in hyphae was examined by FITC‐phalloidin staining of formaldehyde fixed hyphae. F‐actin was found to be mainly concentrated in the hyphal tips in which it formed a uniform cap. Apical actin could be involved in hyphal morphogenesis, organelle motility and maintenance of polarity.