Open AccessAdjuvant effects of dimethyl dioctadecyl ammonium bromide, complete Freund's adjuvant and aluminium hydroxide on neutralizing antibody, antibody‐isotype and delayed‐type hypersensitivity responses to Semliki Forest virus in mice
Author(s) -
Katz David,
Lehrer Shoshana,
Galan Orna,
Lachmi BatEl,
Cohen Shoshana
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04255.x
Subject(s) - antibody , adjuvant , semliki forest virus , virology , virus , neutralizing antibody , isotype , chemistry , biology , microbiology and biotechnology , immunology , monoclonal antibody , biochemistry , rna , gene
Summary Outbred mice were inoculated subcutaneously with inactivated Semliki Forest virus (SFV) in saline and combinations of the virus with complete Freund's adjuvant (CFA) aluminium hydroxide (Al) and dimethyl dioctadecyl ammonium bromide (DDA). The immune response was evaluated for delayed‐type hypersensitivity, for total ELISA antibodies and antibody‐isotypes and for neutralizing antibodies. DDA was the most efficient adjuvant in inducing DTH, CFA the second and Al induced a DTH response that was only slightly higher (statistically not significant) than that induced by the inactivated virus without adjuvants. All adjuvants enhanced the production of ELISA antibodies to similar levels. However, the levels of neutralizing antibodies induced were low in mice which were inoculated with the inactivated SFV alone or mixtures of the virus with Al. DDA induced high levels of neutralizing antibodies and CFA induced intermediate levels. The pattern of antibody‐isotypes induced by DDA and CFA was different from the pattern induced by the inactivated virus or by the virus mixed with Al: DDA and CFA induced low amounts of IgG 1 antibodies and relatively higher amounts of IgG 2a and IgG 2b antibodies while the inactivated virus and the mixture of the virus with Al induced higher proportions of IgG 1 antibodies. In sera from convalescent mice the majority of antibody activity resided in the IgG 2a and IgG 2b immunoglobulin subclasses, while IgG 1 antibodies were undetectable.