Open AccessStimulation of human neutrophil phagocytosis of Candida albicans by a β‐glucan synthase inhibitor, cilofungin
Author(s) -
Richardson M.D.,
Smyth L.A.,
Shankland G.S.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04226.x
Subject(s) - candida albicans , phagocytosis , microbiology and biotechnology , complement system , antibody opsonization , corpus albicans , glucan , alternative complement pathway , biology , chemistry , immunology , biochemistry , antibody , opsonin
Summary The effect of a lipopeptide antifungal agent, cilofungin, on serum opsonization and phagocytosis of Candida albicans yeast phase cells in human neutrophil monolayer assays was investigated. Simultaneous addition of fungicidal concentrations of cilofungin did not enhance or inhibit phagocytosis of C. albicans . Pretreatment of Candida blastospores with cilofungin in the absence of serum complement for 1 h did not affect phagocytosis. However, pretreatment of blastospores with cilofungin and complement promoted a significant increase in ingestion. Pretreatment of neutrophils with cilofungin in serum‐free media did not affect neutrophil viability. In contrast, pre‐exposure of neutrophils to cilofungin in the presence of complement inhibited ingestion of blastospores.