Open AccessStimulation of antimycobacterial activity in mouse peritoneal macrophages by priming with trehalose dimycolate (TDM)
Author(s) -
Afroum Samia,
Oswald Isabelle P.,
Lantier Frédéric,
Petit JeanFrançois,
Lemaire Geneviève
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04222.x
Subject(s) - antimycobacterial , microbiology and biotechnology , lipopolysaccharide , mycobacterium tuberculosis , in vitro , priming (agriculture) , stimulation , biology , mycobacterium , macrophage , immunology , bacteria , medicine , tuberculosis , biochemistry , pathology , botany , germination , genetics , neuroscience
Summary We examined the potential of two bacterial immunomodulators, trehalose dimycolate (TDM) and lipopolysaccharide (LPS), to stimulate the capacity of mouse peritoneal macrophages to control the growth of the intracellular bacterium, Mycobacterium tuberculosis BCG. Macrophages were obtained from mice innately susceptible ( Bcg s ) or resistant ( Bcg r ) to BCG infection. In all mouse strains tested ( Bcg r and Bcg s ), with the exception of BALB/c ( Bcg s ), TDM was sufficient to elicit macrophages with strong antimycobacterial activity in vitro. In BALB/c mice, the induction of anti‐BCG activity required two signals, TDM and LPS, given in sequence. Our data suggest that additional gene(s), besides the Bcg locus, control macrophage resistance to BCG.