Open AccessRelationship of the chemical structure and immunobiological activities of lipoteichoic acid from Streptococcus faecalis ( Enterococcus hirae ) ATCC 9790
Author(s) -
Tsutsui Osamu,
Kokeguchi Susumu,
Matsumura Tomohiro,
Kato Keijiro
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04217.x
Subject(s) - enterococcus hirae , lipoteichoic acid , enterococcus faecalis , microbiology and biotechnology , chemistry , streptococcaceae , teichoic acid , enterococcus , biochemistry , bacteria , biology , enzyme , escherichia coli , staphylococcus aureus , antibiotics , peptidoglycan , genetics , gene
Summary Two molecular species of lipoteichoic acid (LTA 1 and LTA 2) were isolated from whole cells of Streptococcus faecalis ( Enterococcus hirae ) ATCC 9790 by hydrophobic chromatography on Octyl Sepharose CL‐4B. Chemical analysis revealed that LTA 1 and LTA 2 contained two and four acyl lipid anchors respectively. LTA 1 was less active than LTA 2 in inducing cytokines, except interleukin‐1 (IL‐1), but their in vivo antitumour effects were similar. LTA 2 was a potent inducer of tumour necrosis factor (TNF) and interferon (IFN) production and had excellent antitumour activity against Meth A fibrosarcoma established in mice. Deacylation of LTA 2 by alkaline hydrolysis abolished these biological activities. The phosphatidylglycolipid fraction derived from LTA 2 after acid hydrolysis could also induce TNF, IFN, and IL‐1 production, as well as having antitumour activity against Meth A fibrosarcoma. Therefore, the lipid anchor portion of S. faecalis LTA may play an important role in the manifestation of these various biological activities.