Open AccessThe use of synthetic O‐antigens of Salmonella for improvement of serological diagnosis of enteric infections
Author(s) -
Tendetnik Yuri Ya.,
Pokrovsky Valentin I.,
Chernyak Anatoliy Ya.,
Levinsky Anatoliy B.,
Kochetkov Nikolay K.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04202.x
Subject(s) - antigen , salmonella , hemagglutination , immunodiffusion , serology , microbiology and biotechnology , hemagglutination assay , antibody , radial immunodiffusion , ouchterlony double immunodiffusion , biology , virology , immunology , bacteria , antiserum , genetics , titer
Summary Synthetic Salmonella O‐antigens of a new copolymeric type, unlike natural antigens (lipopolysaccharides), exhibit monospecificity for groupspecific factors 0:3, 0:4, and 0:9 of Salmonella serogroups E, B, and D, and are, by 1–2 orders of magnitude, more sensitive in double immunodiffusion and passive hemagglutination inhibition tests. The use of the synthetic antigens for detection of antibodies in patients' sera by means of passive hemagglutination increased the specificity of this test considerably, thereby improving immunodiagnosis of salmonellosis.