Open AccessDetection of common polysaccharide antigen of Pseudomonas aeruginosa with O‐antiserum to Pseudomonas cerasi
Author(s) -
Kocharova Nina A.,
Knirel Yuriy A.,
Romanowska Anna,
Romanowska Elzbieta,
Makarenko Tatyana A.,
Edvabnaya Liliya S.,
Stanislavsky Evgeny S.,
Zdorovenko Galina M.,
Yakovleva Lyudmila M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04199.x
Subject(s) - pseudomonas aeruginosa , antiserum , antigen , microbiology and biotechnology , polysaccharide , pseudomonas , lipopolysaccharide , pseudomonadaceae , pseudomonadales , chemistry , bacteria , biology , biochemistry , immunology , genetics
Summary The identity of the structures of common polysaccharide antigen (CPA) of Pseudomonas aeruginosa and O‐antigen of Pseudomonas cerasi was used for immunochemical study of polysaccharide antigens of seven immunotypes (IT) of P. aeruginosa . ELISA performed with O‐antiserum to P. cerasi showed that CPA is present in all seven ITs in different amounts. In SDS‐PAGE this antigen behaves as a lipopolysaccharide (LPS) and is detected by immunoblotting technique in five of seven ITs.