Open AccessSynergistic effect on blastogenesis in murine spleen cells of lipopolysaccharide, lipid A, and acid‐degraded polysaccharide from Fusobacterium nucleatum
Author(s) -
Sveen Kjell,
Hofstad Tor
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04162.x
Subject(s) - spleen , lipopolysaccharide , fusobacterium nucleatum , lipid a , pokeweed mitogen , microbiology and biotechnology , biology , polysaccharide , cell culture , cell , chemistry , biochemistry , bacteria , immunology , in vitro , peripheral blood mononuclear cell , porphyromonas gingivalis , genetics
Summary Interchangeable combinations of Fusobacterium nucleatum Fev1 lipopolysaccharide (LPS) with its split products by acetic acid hydrolysis, i.e. lipid A (LA) and degraded polysaccharide (PS), amplified the blastogenic response in murine spleen cell cultures as measured by [ 3 H]thymidine uptake. Athymic murine spleen cells precultured with LPS‐Fev1 for 48 h (stage 1), washed twice and cultured together with fresh cells and either LA or PS for 72 h (stage 2) gave a synergistic response over that found in spleen cell cultures of thymic mice. Spleen cells pre‐cultured with LA or PS and with fresh cells and LPS‐Fev1 added to stage 2 cultures gave less significant amplification compared with precultures of LPS and either LA or PS together with fresh cells added to stage 2. Precultures with LA, PS or LPS‐Fev1 and with pokeweed mitogen (PWM) and fresh cells added produced an additional increment of synergy which was most pronounced in spleen cell cultures of normal mice.