Open AccessThe construction of a monoclonal diagnostic system for the field detection of Vibrio cholerae
Author(s) -
AlRiyami Asya,
Haynes Lorraine G.,
Campbell Ailsa M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04159.x
Subject(s) - vibrio cholerae , microbiology and biotechnology , vibrionaceae , biology , bacteria , genetics
Summary An enzyme‐based double monoclonal field diagnostic system detecting both serotypes of Vibrio cholerae has been developed. The system uses nitrocellulose as a solid support, 1.25% skimmed dried milk as blocking reagent, water as washing reagent, and alkaline phosphatase cross‐linked to antibody by means of glutaraldehyde as detecting reagent. The sensitivity of the system was 10 5 vibrios/ml. The biotin‐avidin system gave sensitivity an order of magnitude weaker. There were no cross‐reactions with the range of other bacteria tested.