Open AccessDNA homology comparison between American and European Borrelia burgdorferi strains
Author(s) -
Fattorini P.,
Graziosi G.,
Balanzin D.,
Cinco M.
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04157.x
Subject(s) - southern blot , borrelia burgdorferi , biology , restriction enzyme , strain (injury) , dna , homology (biology) , dna–dna hybridization , nucleic acid thermodynamics , microbiology and biotechnology , restriction map , northern blot , genetics , virology , gene , nucleic acid sequence , base sequence , rna , antibody , anatomy
Summary Seven strains of Borrelia burgdorferi isolated from ticks and from human beings in Europe and U.S.A. were analyzed for DNA restriction patterns with several enzymes and for DNA homology in Southern blot hybridizations. The restriction patterns showed a moderately high variability. In Southern blot hybridization, strain B31 (U.S.A.) DNA gave a strong signal with itself, strains Bsf (U.S.A.) and Alcaide (isolated in Italy but presumably contracted in Venezuela). Strain B45 (F.R.G.) hybridized to itself, strain BITS (Italy) and to strain D.A. (Italy). Strain Nancy (Italy) gave a signal only when hybridized to itself, although it was classified as Borrelia on the basis of the clinical manifestations, SDS‐PAGE protein pattern and antigenic determinants. No hybridization differences were observed for strains isolated from different hosts in the same continental geographical area.