Open AccessSaccharide‐protein covalent conjugates: immunochemical characterization of Citrobacter 036 core oligosaccharide‐tetanus toxoid conjugates
Author(s) -
Ługowski Czesław,
Kułakowska Małgorzata,
Romanowska Elżbieta
Publication year - 1991
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1991.tb04155.x
Subject(s) - toxoid , conjugate , tetanus , conjugate vaccine , chemistry , oligosaccharide , epitope , lipopolysaccharide , citrobacter , antigen , antiserum , immunization , microbiology and biotechnology , biochemistry , biology , escherichia coli , virology , enterobacteriaceae , immunology , vaccination , mathematical analysis , mathematics , gene
Summary Core oligosaccharides (complete and incomplete) isolated from Citrobacter 036 lipopolysaccharide were covalently conjugated with tetanus toxoid. Serological examination of the Citrobacter 036 core oligosaccharide‐tetanus toxoid conjugates showed that they are strong immunogens. The monospecific anti‐conjugate sera prepared by immunization of rabbits, were used to study the antigenic relations between lipopolysaccharide core regions of 8 strains of Citrobacter . Immunoelectrophoresis, immunoblotting and quantitative microprecipitation were performed in the experiments.