Open AccessImmunological evidence for a ribulose bisphosphate carboxylase‐like protein in the symbiotic dinoflagellate Symbiodinium sp.
Author(s) -
Gerberding Holger,
McNeil Yvette,
Yellowlees David,
Miller David J.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb14001.x
Subject(s) - dinoflagellate , ribulose 1,5 bisphosphate , symbiodinium , rubisco , pyruvate carboxylase , protein subunit , biology , biochemistry , botany , photosynthesis , enzyme , bacteria , genetics , symbiosis , gene
1. Summary Whereas previously there has been no convincing evidence for ribulose bisphosphate carboxylase in dinoflagellates, a strong and highly specific reaction was observed when antibodies to the denatured large subunit of the (silver beet) protein were used to probe Western blots of whole soluble fractions of various Symbiodinium isolates. No reaction was observed using extracts from Symbiodinium isolated from a host which had been maintained under low light intensity. The results imply extensive sequence homology between the large subunit of ribulose bisphosphate carboxylase and a dinoflagellate protein of M , approximately 35 000.