Open AccessEfficient cloning in Bacillus megaterium : comparison to Bacillus subtilis and Escherichia coli cloning hosts
Author(s) -
Tersch Michael A.,
Robbins Helen Loidl
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13994.x
Subject(s) - bacillus megaterium , bacillus subtilis , escherichia coli , transformation (genetics) , cloning (programming) , plasmid , biology , bacillaceae , insert (composites) , microbiology and biotechnology , recombinant dna , bacteria , gene , genetics , materials science , computer science , composite material , programming language
1. Summary Quantitative cloning efficiencies for B. megaterium, B. subtilis , and E. coli were compared. Transformation of B. megaterium is less efficient than transformation of B. subtilis or E. coli . The frequency of recombinant clones was equal in E. coli and B. megaterium ; both somewhat higher than in B. subtilis . Equivalent average insert sizes were found in B. megaterium and E. coli clones, but significantly smaller inserts were obtained in B. subtilis clones. Clones obtained and propagated in B. megaterium were structurally stable when grown under plasmid selection.