Open AccessDistribution of transition metal ions in multiple forms of Methanosarcina hydrogenase
Author(s) -
Bhosale S.B.,
Yeole T.Y.,
Kshirsagar D.C.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13985.x
Subject(s) - hydrogenase , methanosarcina barkeri , strain (injury) , methanosarcina , chemistry , transition metal , bacteria , metal , biochemistry , enzyme , biology , archaea , catalysis , methanogenesis , organic chemistry , anatomy , gene , genetics
1. Summary There were significant levels of in vitro hydrogenase activity in Methanosarcina strains. The multiple forms of hydrogenase were observed in cell free extracts of cells grown on methanol. Strains having poor growth on H 2 : CO 2 had four forms while strains having normal growth on all substrates contained two forms of hydrogenase. These multiple forms differ in their charges as well as in their composition of transition metal ions. The strain having normal growth showed higher incorporation of 63 Ni 2+ and 65 Zn 2+ . Both hydrogenases, A and D, of strain P 3 had methylviologen and F 420 ‐reducing activity and contained Zn 2+ and Co 2+ respectively. Hydrogenases A and D of strains P 1 and P 4 also had similar characteristics whereas hydrogenases B and C had only methylviologen reducing activity.