Open AccessScreening of obligate methanotrophs for soluble methane monooxygenase genes
Author(s) -
Stainthorpe A.C.,
Salmond G.P.C.,
Dalton H.,
Murrell J.C.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13980.x
Subject(s) - methane monooxygenase , gene , monooxygenase , obligate , methanotroph , biochemistry , biology , enzyme , genetics , dna , chemistry , anaerobic oxidation of methane , botany , cytochrome p450 , catalysis
1. Summary A 5.8 kb fragment of chromosomal DNA from Methylococcus capsulatus (Bath) containing genes encoding the soluble methane monooxygenase enzyme complex was used as a probe for the detection of soluble monooxygenase genes in a number of representative strains of obligate methanotrophs. Only type II methanotrophs of the genus Methylosinus were found to contain homologues to the Methylococcus gene probe. This probe was also used successfully to detect soluble methane monooxygenase genes in a variety of methanotrophs by colony hybridizations.