Open AccessEvidence for two fatty alcohol oxidases in the biosurfactant‐producing yeast Candida (Torulopsis) bombicola
Author(s) -
Hommel Rolf,
Ratledge Colin
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13975.x
Subject(s) - yeast , alcohol , chemistry , fatty alcohol , biochemistry , fatty acid , enzyme , ethanol , oxidase test , glycolipid , organic chemistry
1. Summary Fatty alcohol oxidase activities in Candida ( Torulopsis ) bombicola ATCC 22214, which produces large amounts of glycolipids consisting of ω 0 and ( ω − 1)‐hydroxyfatty acids and a sugar moiety, occurred only in the microsomal fraction whether the cells had grown on n‐alkanes, carbohydrates or a mixture of the two. High activities occurred in glucose‐grown cells. Aliphatic alcohols from octanol to hexadecanol were oxidized with two maxima in activities for decanol and for tetradecanol. Differences in their pH optima and in temperature stability suggest two separate enzymes are present. Long chain diols, but not ω ‐hydroxyfatty acids, were also oxidized.