Open AccessNucleotide sequence of the G 6 ‐amylase gene from alkalophilic Bacillus sp. H‐167
Author(s) -
Shirokizawa Osamu,
Akiba Teruhiko,
Horikoshi Koki
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13965.x
Subject(s) - gene , amylase , nucleic acid sequence , genetics , sequence (biology) , biology , nucleotide , chemistry , microbiology and biotechnology , biochemistry , enzyme
1. Summary The nucleotide sequence of the G 6 ‐amylase gene from alkalophilic Bacillus sp. H‐167 was determined. The open reading frame of the gene consisted of 2865 base pairs, encoding 955 amino acids. The NH 2 ‐terminal amino acid sequence analysis of the G 6 ‐amylase indicated that the enzyme had a single peptide of 33 amino acid residues and the mature enzyme was composed of 922 amino acids, giving a molecular mass of 102598. Identity of the NH 2 ‐terminal amino acid sequences among each component of the multiform G 6 ‐amylase suggested the proteolytic processing of the COOH‐terminal side of the enzyme. The DNA sequence and the deduced amino acid sequence of the G 6 ‐amylase gene showed no homology with those of other bacterial α‐amylases although the consensus amino acid sequences of the active center were well conserved.