Open AccessNucleotide sequence of the fosB gene conferring fosfomycin resistance in Staphylococcus epidermidis
Author(s) -
Zilhao Rita,
Courvalin Patrice
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13949.x
Subject(s) - nucleic acid sequence , biology , plasmid , gene , genetics , homology (biology) , peptide sequence , fosfomycin , open reading frame , staphylococcus epidermidis , start codon , microbiology and biotechnology , structural gene , coding region , escherichia coli , nucleotide , bacteria , staphylococcus aureus , antibiotics
1. Summary We have determined the nucleotide sequence of gene fosB of plasmid pIP1842, which confers resistant to fosfomycin in Staphylococcus epidermidis BM2641. The resistance gene was identified as a coding sequence of 417 bases pairs corresponding to a protein with a calculated Mw of 16 345 daltons. This value is in good agreement with that of 15 000, estimated by SDS‐polyacrylamide gel electrophoresis of a bacterial cell‐free coupled transcription‐translation system. The fosB gene product exhibits 48% sequence homology with the fosfomycin resistance protein FOSA of Enterobacteriaceae. The substitutions are scattered throughout indicating that the two corresponding genes have diverged from a common ancestor. Downstream from fosB is located an open reading frame that was partially sequenced. The deduced amino‐acid sequence was 50% homologous to REP α1, a protein involved in the replication of the Enterococcus faecalis plasmid pAMα1.