Open AccessTranscriptional control of SSL1, a gene controlling α ‐specific inactivation of a ‐factor in Saccharomyces cerevisiae
Author(s) -
Steden Martin,
Duntze Wolfgang
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13942.x
Subject(s) - saccharomyces cerevisiae , genetics , gene , biology
1. Summary The SSL1 gene of S. cerevisiae which is involved in the cell‐type specific recovery of mating type α cells from cell cycle arrest by the mating hormone a ‐factor has been cloned as a 1 kb Sau 3A fragment in a complementing plasmid from a library of S. cerevisae genomic DNA. Disruption of this gene in wild‐type α ‐cells results in the ssl1 − phenotype which is characterized by an extreme sensitivity to a ‐factor. Transcription analysis revealed that the SSL1 gene is transcribed only in MAT α cells but not in MAT a or diploid (MAT a /MAT α ) cells defining it as a member of the set of α ‐specific genes.