Open AccessComparison of promoter activities in Escherichia coli and Pseudomonas aeruginosa : use of a new broad‐host‐range promoter‐probe plasmid
Author(s) -
Lodge Julia,
Williams Roy,
Bell Andrew,
Chan Bernard,
Busby Stephen
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13867.x
Subject(s) - promoter , escherichia coli , plasmid , biology , operon , pseudomonas aeruginosa , multiple cloning site , microbiology and biotechnology , dna , transcription (linguistics) , genetics , recombinant dna , gene , bacteria , expression vector , gene expression , linguistics , philosophy
The broad‐host‐range plasmid, pRW2, is a derivative of pRK 2501 carrying the Escherichia coli lac operon without a promoter, downstream of a polylinker sequence. We have cloned a number of DNA fragments carrying promoters into this plasmid and measured promoter activity in both E. coli and Pseudomonas aeruginosa . Promoters carrying consensus −10 and −35 sequences were active in both backgrounds and the depencence of activity on the nucleotide sequence of the 35 region was the same in both cases. We also measured the activity of two promoters at which transcription in E. coli was totally dependent on the E. coli activators CRP and FNR: both promoters were found to be ative in P. aeruginosa .