Open AccessIsolation and characterization of an Azotobacter chroococcum mutant deficient in nitrate transport
Author(s) -
Ruiz M.T.,
Cejudo F.J.,
MuñozCenteno M.C.,
Paneque A.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13865.x
Subject(s) - azotobacter chroococcum , nitrate reductase , nitrite reductase , mutant , nitrate , biochemistry , nitrogenase , strain (injury) , microbiology and biotechnology , nitrite , chlorate , chemistry , mutagenesis , azotobacter , biology , bacteria , enzyme , nitrogen fixation , genetics , inorganic chemistry , organic chemistry , anatomy , gene
A chlorate‐resistant Azotobacter chroococcum strain, which we designate TR1, has been isolated after mutagenesis of the parent strain with ICR‐191. After growth in a medium containing nitrate, the mutant strain exhibited activities of nitrogenase, nitrate reductase and nitrise reductase, and it did not take up nitrate efficiently. Induction of nitrite reductase in the mutant required a nitrate concentration higher than in the wild type. The results suggest that TR1 is not a regulatory mutant but a strain deficient in the process of nitrate transport into the cell.