Open AccessAn improved method for measuring the interseptal spacing in hyphae of Streptomyces tendae by fluorescence microscopy coupled with image processing
Author(s) -
Reichl U.,
Yang H.,
Gilles E.D.,
Wolf H.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13864.x
Subject(s) - hypha , fluorescence microscope , visualization , digital image analysis , stain , morphology (biology) , streptomyces , image processing , fluorescence , biological system , chemistry , biology , artificial intelligence , optics , computer science , image (mathematics) , staining , computer vision , microbiology and biotechnology , physics , bacteria , genetics
Improved stain for visualization of septa in streptomycetes is presented. The technique preserves the true morphology of hyphae and enables its arrangement to be interpreted correctly. Using a digital image processing system, accurate and reproducible analysis of positional information on septa and branches was obtained.