Open AccessCloning and expression of the dermonecrotic toxin gene of Pasteurella multocida ssp. multocida in Echerichia coli
Author(s) -
Kamps Aleida M.I.E.,
Kamp Elbarte M.,
Smits Mari A.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13860.x
Subject(s) - pasteurella multocida , biology , antiserum , cloning (programming) , microbiology and biotechnology , gene , toxin , recombinant dna , dna , strain (injury) , molecular cloning , bacteria , genetics , gene expression , antibody , anatomy , computer science , programming language
A DNA library of Pasteurella multocida ssp. multocida strain CVI 47459 was constructed in the LambdaGEM‐11 vector. Recombinant clones that encoded dermonecrotic toxin (DNT) were identified immunologically with antiserum raised against purified DNT. By comparing the DNA restriction maps the DNT gene. Hybridization studies with 10 strains of P. multocida ssp. Multocida suggested that strains that do not produce the DNT do not contain sequences homologous to the DNT gene.