Open AccessGenetic transformation of intact cells of Bacillus subtilis by electroporation
Author(s) -
Brigidi P.,
Rossi E.,
Bertarini M.L.,
Riccardi G.,
Matteuzzi D.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13850.x
Subject(s) - electroporation , bacillus subtilis , plasmid , protoplast , transformation (genetics) , transformation efficiency , biology , bacillaceae , microbiology and biotechnology , bacillales , dna , chemistry , bacteria , biophysics , genetics , gene , agrobacterium
Plasmids DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent‐cell or protoplast transformation. The maximum electroporation efficiency was 10 4 transformants/μg DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformations efficiency.