Open AccessRapid procedure to determine the DNA base composition from small amounts of Gram‐positive bacteria
Author(s) -
Ezaki T.,
Saidi S.M.,
Liu SL.,
Hashimoto Y.,
Yamamoto H.,
Yabuuchi E.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13848.x
Subject(s) - bacteria , gram , cytosine , guanine , lysis , gram positive bacteria , dna , chromatography , gram negative bacteria , composition (language) , chemistry , biology , microbiology and biotechnology , biochemistry , escherichia coli , nucleotide , genetics , gene , linguistics , philosophy
A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram‐positive bacteria is described. Cells of Gram‐positive bacteria were lysed with achromopeptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram‐positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.