Open AccessPlasmid profiles and genomic DNA restriction endonuclease patterns of 30 independent Staphylococcus lugdunensis strains
Author(s) -
Etienne Jerome,
PoitevinLater Françoise,
Renaud François,
Fleurette Jean
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13843.x
Subject(s) - staphylococcus lugdunensis , extrachromosomal dna , plasmid , biology , ecori , restriction enzyme , ribotyping , genetics , microbiology and biotechnology , dna , genomic dna , restriction fragment , restriction map , psti , staphylococcus , staphylococcus aureus , bacteria , polymerase chain reaction , gene
Extrachromosomal DNA analysis and restriction endunuclease analysis of whole cellular DNA were used to characterize 30 Staphylococcus lugdunensis strains isolated from 13 different hospitals from 1977 to 1988. All the strains were susceptible to most of the antibiotics tested, including penicillin G. A single 3.2 kilobase plasmid was detected in 13 strains and one or two plasmids, ranging from 2.3 to 6.6 kilobases, were found in 7 strains. Eco RI, Pst I and Pvu II restriction patterns of total cellular DNA were identical for 23 isolates, indicating strong conservation of endonuclease sites in this species. One or two additional DNA bands occurred in seven isolates. Molecular markers show rather little variations between different S. lugdunensis isolates suggesting that they are closely related.