Open AccessCharacterization of adhesion zones in E. coli cells
Author(s) -
Leduc M.,
Frehel C.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13832.x
Subject(s) - plasmolysis , adhesion , staining , peptidoglycan , escherichia coli , ultrastructure , bacteria , chemistry , stain , negative stain , electron microscope , microbiology and biotechnology , biology , cell wall , biochemistry , anatomy , physics , organic chemistry , gene , optics , genetics
After plasmolysis of Escherichia coli cells, the adhesion zones were characterized using the cytochemical PTA and SP procedures which stain peptidoglycan and lipopolysaccharides (LPS) respectively. A PTA‐stained layer was detected at the adhesion sites. This layer was visualized irrespective of the electron microscopy procedure used. Also, using SP staining an outer membrane in which LPS molecules were asymmetrically distributed, was observed.