Open AccessDifferentiation of Leuconostoc species by nicotinamide adenine dinucleotide‐dependent d (−)‐lactic dehydrogenase profiles
Author(s) -
Dicks L.M.T.,
Vuuren H.J.J.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb13827.x
Subject(s) - leuconostoc mesenteroides , leuconostoc , strain (injury) , polyacrylamide gel electrophoresis , biology , biochemistry , bacteria , chemistry , lactic acid , enzyme , genetics , anatomy
The electrophoretic mobility of the d (−)‐nLDH's of Leuconostoc oenos (nine strains), Leuconostoc mesenteroides subsp. mesenteroides (one strain), Leuconostoc mesenteroides subsp. dextranicum (one strain), Leuconostroc lactis (three strains), Leuconostoc paramesenteroides (two strains) and a Leuconostoc sp. were compared using conventional and stacked polyacrylamide gels. A singel diffused d (−)‐nLDH band was obtained for each strain on a 7% acrylamide gel. However, several well defined d (−)‐nLDH bands were obtained for each strain when a polyacrylamide stacked gel was used. Based on d (−)‐nLDH profiles of strains of L. oenos were different from those obtained for the non‐acidophilic Leuconostoc spp. Leuconostoc mesenteroides subsp. mesenteroides and L. mesenteroides subsp. dextranicum had similar d ‐nLDH profiles. The overall d (−)‐nLDH profiles of L. lactis corresponded well. Different d (−)‐nLDH profiles were obtained for L. paramesenteroides DSM 20201 and the type strain of L. paramesenteroides (DSM 20288 T ). The d (−)‐nLDH profile of Leuconostoc sp. ATCC 21436 corresponded well with the profile obtained for the d (−)‐nLDH electrophoretic marker of Lactobacillus leichmannii .