Open AccessGenetics of CO 2 fixation in the chemoautotroph Alcaligenes eutrophus
Author(s) -
Bowien B.,
Windhövel U.,
Yoo J.G.,
Bednarski R.,
Kusian B.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04951.x
Subject(s) - regulon , operon , biology , genetics , gene , gene cluster , genome , carbon fixation , chromosome , regulation of gene expression , biochemistry , escherichia coli , photosynthesis
The genome of Alcaligenes eutrophus H16 contains two functional, highly homologous clusters of genes ( cfx genes) for at least six Calvin cycle enzymes, with one cluster located on the chromosome and the other on megaplasmid pHG1. The genes within each cluster presumably belong to a single large operon of about 11 kilobase pairs (kb) in size. Both operons form a cfx regulon that is controlled by the chromosomally encoded regulatory gene cfxR . The product of cfxR seems to be a transcriptional activator. Occurrence of isoenzymes within the central carbon metabolism of the organism is a consequence of this unusual genetic setup.