Open AccessInfluence of respiratory substrate on the cytochrome content of Shewanella putrefaciens
Author(s) -
Morris C.J.,
Gibson D.M.,
Ward F.B.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04241.x
Subject(s) - shewanella putrefaciens , cytochrome , trimethylamine , chemistry , electron acceptor , reductase , substrate (aquarium) , biochemistry , shewanella oneidensis , cytochrome c oxidase , chromatography , bacteria , biology , enzyme , ecology , genetics
Shewanella putrefaciens can use trimethylamine oxide (TMAO) as electron acceptor under anoxic conditions. The associated cytochromes induced during growth under various respiratory conditions have been separated by liquid chromatography (DEAE Sepharose CL7b) and SDS‐PAGE and characterized spectrophotometrically and by redox potentiometry. Two major low potential cytochromes and at least three minor low potential cytochromes, likely to be involved in TMAO reduction, were found. No cytochrome specific for TMAO reductase was found.