Open AccessThe effect of nuclease on transformation efficiency in Serratia marcescens
Author(s) -
Palomar Julio,
Guasch Joan Francesc,
Regué Miquel,
Viñas Miquel
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04240.x
Subject(s) - serratia marcescens , nuclease , transformation (genetics) , plasmid , microbiology and biotechnology , escherichia coli , recombinant dna , biology , transformation efficiency , extracellular , bacteria , enterobacteriaceae , dna , genetics , gene , agrobacterium
No differences in the efficiency of transformation were observed from both plasmid and chromosomal DNA in Serratia marcescens 2170 and and extracellular nuclease defective isogenic strain. The efficiency of transformation was the same for Escherichia coli 5K and E. coli containing a recombinant plasmid conferring the ability to synthesize a S. marcescens nuclease. From these results we conclude that the extracellular nuclease of S. marcescens 2170 is not the main cause of the low efficiency of transformation observed in this bacterium.