Open AccessAnaerobic 109 Cd accumulation by cadmium‐resistant and ‐sensitive Staphylococcus aureus
Author(s) -
Tynecka Zofia,
Skwarek Teresa,
Malm Anna
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04193.x
Subject(s) - nigericin , efflux , cadmium , staphylococcus aureus , anaerobic exercise , chemistry , strain (injury) , phosphate , microbiology and biotechnology , nuclear chemistry , biochemistry , bacteria , biophysics , biology , membrane , anatomy , organic chemistry , physiology , genetics
Washed cells of the cadmium‐sensitive Staphylococcus aureus 17810S accumulated 109 Cd under anaerobic conditions via the Mn 2+ porter down ΔΨ in 1 or 100 mM phosphate buffer, pH 7; in washed cells of the cadmium‐resistant S. aureus 17810R 109 Cd accumulation was highly reduced. Nigericin did not stimulate anaerobic Cd 2+ accumulation by strain 17810R in 100 mM phosphate buffer, suggesting that ΔΨ could energize Cd 2+ efflux. In 1 mM phosphate buffer nigericin restored Cd 2+ accumulation via the Mn 2+ porter down ΔΨ in strain 17810R, indicating involvement of ΔΨ in Cd 2+ extrusion. Increase of phosphate buffer concentration from 1 to 100 mM and addition of energy source at steady‐state caused ΔΨ‐dependent Cd 2+ efflux from the nigericin‐pretreated cells of strain 17810R. This suggests that the Cd 2+ efflux system in S. aureus may require energy of both ATP and Δμ H .