Open AccessBiochemical and genetic analysis of an alpha‐mannosidase mutant from Saccharomyces cerevisiae
Author(s) -
Cueva Rosario,
Bordallo Carmen,
Rendueles Paz Suárez
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04192.x
Subject(s) - mutant , biology , mannosidase , saccharomyces cerevisiae , yeast , gene , mutation , genetics , microbiology and biotechnology , mannose , biochemistry
A yeast mutant lacking non‐specific α‐mannosidase activity was found as a background marker during our search for dap2 mutants (Suárez‐Rendueles, P. and Wolf, D.H. (1987) J. Bacteriol. 169, 4041–4048). The mutant (DPS‐15) is characterized in detail. The mutation called amd1 segregates 2:2 in meiotic tetrads, indicating a single chromosomal gene mutation which is recessive. Diploids heterozygous for amd1 show gene dosage. Thus, it appears that AMD1 might be the structural gene for α‐mannosidase. Results obtained with this mutant show that α‐mannosidase is not a vital component of the vegetative cell cycle. The differentiation process of sporulation is not disturbed in homozygous mutant diploids. Mannose turnover does not seem to be altered in mutant cells.