Open AccessIndication of a modified EMP pathway for fructose breakdown in a halophilic archaebacterium
Author(s) -
Altekar Wijaya,
Rangaswamy Vidhya
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04190.x
Subject(s) - halophile , fructose , chemistry , biochemistry , biology , bacteria , genetics
The breakdown of fructose in Haloarcula (Halobacterium) vallismortis probably takes place via a novel modification of Embden Meyerhof‐Parnas (EMP) pathway. Phosphorylation of fructose to yield frutose 1‐phosphate as the product appears to be the initial step in fructose utilization. The noteworthy feature is that fructose 1‐phosphate formation occurs by an ATP‐dependent fructose 1‐phosphotransferase (ketohexokinase) rather than by the phosphoenolpyruvate‐dependent fructose phosphotransferase system, commonly found in eubacteria. Ketohexokinase activity has so far been known to occur only in eukaryotes. Fructose 1‐phosphate was converted into fructose 1,6‐biphosphate by the action of 1‐phosphofructokinase. Ketohexokinase and 1‐phosphofructokinase activities have been demonstrated for the first time in any archaebacterium.