Open AccessBiotinylated oligonucleotide probes for the detection and the characterization of TEM‐type extended broad spectrum β‐lactamases in enterobacteriaceae
Author(s) -
Tham T.N.,
Mabilat C.,
Courvalin P.,
Guesdon J.L.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04185.x
Subject(s) - biotinylation , enterobacteriaceae , oligonucleotide , plasmid , biology , microbiology and biotechnology , nucleic acid sequence , imipenem , bacteria , escherichia coli , gene , genetics , antibiotic resistance
Point mutations in the nucleotide sequence of the structural genes for the TEM‐type penicillinases can broaded their substrate spectrum towards all β‐lactams except cephamycins and imipenem. The presence of such variants on self‐transferable plasmids accounts for the dissemination of this new type of resistance to numerous species of Enterobacteriaceae in various countries. We have synthetized biotinylated oligonucleotide probes for the detection and the discrimination of parental and mutated nucleotide sequences of TEM enzymes. Seven clinical isolates belonging to four species and harbouring TEM‐1, TEM‐3 or TEM‐6 were studied. The results obtained indicate that detection of TEM‐derived broad spectrum β‐lactamases in clinical isolates of Enterobacteriaceae is possible with biotinylated oligonucleotide probes.