Open AccessA comparison of HEp‐2 cell invasion by enteropathogenic and enteroinvasive Escherichia coli
Author(s) -
Donnenberg Michael S.,
DonohueRolfe Arthur,
Keusch Gerald T.
Publication year - 1990
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1990.tb04179.x
Subject(s) - enteropathogenic escherichia coli , biology , microbiology and biotechnology , escherichia coli , microfilament , gentamicin protection assay , cell , microtubule , cytoskeleton , biochemistry , cancer , genetics , gene , metastasis
In order to further characterize cellular invasion by enteropathogenic Escherichia coli (EPEC), we compared invasion of HEp‐2 cells by EPEC and enteroinvasive E. coli (EIEC). We used a gentamicin HEp‐2 cell assay and measured bacterial recovery under conditions of varying incubation time and temperature, and in the presence or absence of inhibitors of cellular microfilaments and microtubules. We found that, unlike EIEC, EPEC did not rapidly multiply within HEp‐2 cell but invaded well at 32°C. While microfilament inhibitors reduced invasion by both EIEC and EPEC, microtubule inhibitors reduced invasion by EPEC only. These results suggest that EPEC and EIEC differ in their mechanisms of epithelial cell invasion.